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Amphibian Profile

Test number: 8567


    1 ) Batrachochytrium dendrobatidis ( Chytridiomykosis / Chytrid / Bd / Bsal )

Species
Amphibians .
Description

(Batrachochytrium Dendrobatidis)

The chytrid fungus Batrachochytrium (B.) dendrobatidis was first identified in Australia in 1998 and named in 1999. This fungus is thought to be responsible for the population decline and the global extinction of >200 amphibian species. Infections with B. dendrobatidis are in many cases associated with very high mortality rates (in lab up to 100%), but the fungus is not necessarily lethal. Other factors such as stress or co-infections with other pathogens also appear to play a role. B. dendrobatidis multiplies in keratinized tissue and therefore affects primarily the outer skin of adult animals (stratum corneum to the stratum granulosum). In larvae, the horn strips on the mouth are affected. During metamorphosis the infections can lead to dramatic high mortality rates. The clinical symptoms are often nonspecific and may, in addition to the skin (often appear acroscopically unchanged or „blunt“ or depigmented; hyperkeratosis and massive skinning episodes, mixed infections with severe erosions of the skin) or behavioural changes (atypical behaviour, such as prolonged stay in the water, ataxia and CNS problems). Spontaneous deaths without previous overt clinical disease are also observed.

Sample Requirements
Swab, Tissue

Swab without medium: skin swabs of the ventral body surface (adult animals) or rather of the keratinized skin at the mouth (tadpoles), tissue (skin frazzles of infected animals)

Turnaround
1-3 weeks

    2 ) Batrachochytrium salamandrivorans (Chytrid / Bd / Bsal)

Species
Amphibians .
Description

Batrachochytrium salamandrivorans

Batrachochytrium salamandrivorans is a recently described highly-contagious and deadly chytrid fungus that has massively infested and killed fire salamanders especially in North-west Europe. Infected animals show anorexia, apathy and ataxia as well as skin lesions with superficial erosions and deep ulcerations all over the body. Suitable test materials are skin biopsies and swabs.

Sample Requirements
Swab without medium (skin), tissue (skin)
Turnaround
1-3 weeks

    3 ) Rana Virus

Species
Amphibians , Fish , Reptile .
Clinical Signs
Snakes: Hepatitis, stomatitis
Chelonian:Stomatitis, hepatitis
Lizards: Hepatitis, skin lesions
Description

Snakes

Ranaviruses are found in snakes as well as in turtles and tortoises and lizards. They are associated with granulomatous lesions in the liver and the oral cavity. Virus detection is carried out from oral swabs or liver samples.

Chelonian

Ranaviruses are important pathogens in amphibians. In recent years, they have also been increasingly found in reptiles. They have been detected in turtles and tortoises, lizards, and snakes. In chelonians, they can cause stomatitis, hepatitis, and sudden death. The detection of ranaviruses in live animals can be carried out using oral swabs or blood. In dead animals, liver, tongue, and skin have been successfully used for virus detection

Lizards

Ranaviruses are seen in lizards with skin lesions, hepatitis, and sudden death. Virus detection in live animals is carried out using oral swabs or skin. In dead animals, the liver, tongue, and skin can be used.

Sample Requirements
Snakes:Oral and cloacal swabs, liver
Chelonian: Oral and cloacal swabs, blood, tongue, liver
Lizards: Oral and cloacal swabs, skin, liver
Fish: biopsy (gills), blood, tissue (e.g. Liver or kidney), swab (skin)
Turnaround
1-2 weeks
Price

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To order:

  • Download Order Form from this link pdf
  • Complete the order form and send it together with your samples to the following address:

    Laboklin (UK),   Unit 20, Wheel Forge Way, Trafford Park, Manchester, M17 1EH

  • If you have any queries, please contact us on 0161 282 3066 or email us info@laboklin.co.uk
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LABOKLIN GmbH & Co. KG
ISO / DIN 17025 Accredited Laboratory
© 2007-2023 Laboklin (UK)
Unit 20, Wheel Forge Way, Trafford Park, Manchester, M17 1EH
Tel. 0161 282 3066