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Chinese Crested KC DNA bundle (PLL + prcd-PRA)
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1 ) Primary Lens Luxation (PLL)
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Breeds
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American Eskimo
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American Hairless Terrier
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Australian cattle dog
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Chinese Crested
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Danish Swedish Farmdog
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Fox Terrier
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German Hunting Terrier
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Jack Russell Terrier
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Jagd Terrier
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Lakeland Terrier
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Lancashire Heeler
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Lucas Terrier
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Miniature Bull Terrier
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Norfolk Terrier
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Norwich Terrier
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Parson Russell Terrier (PRT)
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Patterdale Terrier
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Pug
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Rat Terrier
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Sealyham Terrier
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Teddy Roosevelt Terrier
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Tenterfield Terrier
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Tibetan Terrier
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Toy Fox Terrier
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Volpino Italiano
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Welsh Terrier
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Westphalia Terrier
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Wire-haired Fox Terrier
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Yorkshire Terrier
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Kennel Club
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This test is part of the Official UK Kennel Club DNA Testing Scheme in Chinese Crested , Jack Russell Terrier , Lancashire Heeler , Miniature Bull Terrier , Parson Russell Terrier (PRT) , Sealyham Terrier , Tibetan Terrier , and Welsh Terrier.
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The Disease |
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The zonula fibres secure the position of the lens. Dogs affected from PLL have painful glaucomas and blindness due to a dislocation of the lens due to a breakdown or disintegration of the zonula fibres. PLL can be inherited or acquired. Therefore the disease might also affect genetically free dogs. First clinical signs of the inherited form of PLL are detectable at the very young age of 20 months. A complete lens luxation typically occurs at the age of 3 to 8 years.
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Trait of Inheritance |
Recently, Cathryn Mellersh and colleagues (Farias et al., 2010) identified a mutation in the gene ADAMTS17 that is responsible for the development of inherited PLL.
The mode of inheritance of PLL is autosomal recessive. This means that PLL-affected dogs receive one mutated gene (allel) from the mother as well as from the father. Hence, the parents need to carry at least one mutated allel.
In most cases heterozygous carriers are healthy. However, it is estimated that about 2 – 20 % of the carriers will develop PLL. |
Inheritance : AUTOSOMAL
RECESSIVE
trait
Sire
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Dam
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Offspring
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clear
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clear
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100% clear
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clear
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carrier
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50% clear + 50%
carriers
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clear
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affected
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100% carriers
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carrier
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clear
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50% clear + 50%
carriers
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carrier
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carrier
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25% clear + 25% affected
+ 50% carriers
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carrier
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affected
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50% carriers + 50%
affected
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affected
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clear
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100% carriers
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affected
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carrier
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50% carriers + 50%
affected
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affected
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affected
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100% affected
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Clear
Genotype: N / N [ Homozygous normal ]
The dog is noncarrier of the mutant gene.
A dog like this is healthy and does not carry the mutated allel responsible for PLL disease. Offspring
of this dog will not get the mutated allel.
Carrier
Genotype: N / PLL [ Heterozygous ]
The dog carries one copy of the mutant gene and one
copy of the normal gene.
The dog has one copy of the normal allel and in addition one copy of the mutated allel. Carriers have
a low risk of developing PLL, however they will pass on the mutation to their offspring. In most cases heterozygous carriers are healthy. However, it is estimated that about 2 – 20 % of the carriers will develop PLL
Affected
Genotype: PLL / PLL [ Homozygous mutant ]
The dog carries two copies of the mutant gene and
therefore it will pass the mutant gene to its entire offspring.
The dog has two copies of the mutated allel. Affected dogs have a high risk of developing PLL during
their lifetime. The mutated allel will be passed to 100% of the offspring. It is recommended to
examine the eyes of genetically affected dogs every 6 months by a specialist in order to detect the
clinical signs of PLL as early as possible.
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. . |
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2 ) Progressive Retinal Atrophy (prcd-PRA): (8094P / 8127)
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Kennel Club: results of this test is accepted by the Kennel Club
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Breeds
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American Cocker Spaniel
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American Eskimo
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Australian cattle dog
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Australian Shepherd
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Australian Stumpy Tail Cattle Dog
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Australian Stumpy tail cattle Dog
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Barbet (French Water Dog)
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Bearded Collie
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Bolognese
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Bolonka Zwetna
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Chesapeake Bay Retriever
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Chihuahua
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Chinese Crested
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Cockapoo (English)
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Cockapoo (American)
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Cocker Spaniel
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Dwarf poodle
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English Cocker Spaniel
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English shepherd
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Entlebuch Mountain dog
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Finnish Lapphund
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German Spitz
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Giant Schnauzer
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Golden Retriever
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Jack Russell Terrier
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Karelian Bear Dog
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Kuvasz
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Labradoodle
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Labrador Retriever
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Lagotto Romagnolo
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Lapponian Herder
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Markiesje
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Miniature Poodle
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Miniature American Shepherd
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Norwegian Elkhound
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Nova Scotia Duck tolling Retriever ( NSDTR )
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Parson Russell Terrier (PRT)
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Poodle
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Portuguese Waterdog
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Schipperke
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Australian Silky Terrier
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Spanish Water Dog
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Standard Poodle
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Swedish Lapp Hund
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Toy Poodle
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Waeller (Wäller)
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Yorkshire Terrier
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Kennel Club
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This test is part of the Official UK Kennel Club DNA Testing Scheme in American Cocker Spaniel , Australian cattle dog , Australian Shepherd , Barbet (French Water Dog) , Chesapeake Bay Retriever , Chinese Crested , Cocker Spaniel , English Cocker Spaniel , Entlebuch Mountain dog , Finnish Lapphund , Giant Schnauzer , Labrador Retriever , Miniature Poodle , Norwegian Elkhound , Nova Scotia Duck tolling Retriever ( NSDTR ) , Portuguese Waterdog , Spanish Water Dog , Standard Poodle , and Toy Poodle.
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The Disease |
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Progressive retinal atrophy (PRA) as an inherited disease occurs in many dog breeds and also in different forms. The form of progressive rod-cone degeneration (prcd-PRA) is a photoreceptor degeneration in dogs with varying ages of onset. This genetic disorder causes the degeneration of retinal cells in the eye: firstly, rod cells are affected, thus leading to progressive night blindness. Secondly, degeneration of the cone cells results in complete blindness of the dog, even in full light situations during the day.
Age of onset of clinical symptoms is typically in early adolescence or early adulthood. However, the onset of the disease may vary among different dog breeds.
Since diagnosis of retinal diseases in dogs may prove difficult, the genetic test on prcd-PRA helps to diagnose a specific disease and is also a useful tool for breeders to eliminate the mutated gene from the dog population.
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Trait of Inheritance |
The mutation in the PRCD gene which has been suggested to cause prcd-PRA has recently been published by the group of Gustavo D. Aguirre at the University of Pennsylvania, USA, and could be found in several dog breeds.
Prcd-PRA is inherited as an autosomal recessive trait. So there are three conditions a dog can be: it can be clear (genotype N/N or homozygous normal) meaning that it does not carry the mutation and will not develop the prcd-form of PRA. Since it also cannot pass the mutation onto its offspring, it can be mated to any other dog.
A dog which has one copy of the PRCD gene with the mutation and one copy without the mutation is called a carrier or heterozygous (genotype N/PRA); while it will not be affected by prcd-PRA, it can pass the mutation onto its offspring and should therefore only be mated to clear dogs.
Dogs that develop this form of PRA have two PRCD gene copies with the mutation (genotype PRA/PRA or homozygous affected); they will always pass the mutated gene onto their offspring and should also be mated only to clear dogs.. |
Inheritance : AUTOSOMAL
RECESSIVE
trait
Sire
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Dam
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Offspring
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clear
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clear
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100% clear
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clear
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carrier
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50% clear + 50%
carriers
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clear
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affected
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100% carriers
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carrier
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clear
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50% clear + 50%
carriers
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carrier
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carrier
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25% clear + 25% affected
+ 50% carriers
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carrier
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affected
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50% carriers + 50%
affected
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affected
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clear
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100% carriers
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affected
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carrier
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50% carriers + 50%
affected
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affected
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affected
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100% affected
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Clear
Genotype: N / N [ Homozygous normal ]
The dog is noncarrier of the mutant gene.
It is very unlikely that the dog will develop Progressive Retinal Atrophy (prcd-PRA): (8094P / 8127). The dog will never pass the mutation to its offspring, and therefore it can be bred to any other dog.
Carrier
Genotype: N / PRA [ Heterozygous ]
The dog carries one copy of the mutant gene and one
copy of the normal gene.
It is very unlikely that the dog will develop Progressive Retinal Atrophy (prcd-PRA): (8094P / 8127) but since it carries the mutant gene, it can pass it on to its offspring with the probability of 50%. Carriers should only be bred to clear dogs. Avoid breeding carrier to carrier because 25% of their offspring is expected to be affected (see table above)
Affected
Genotype: PRA / PRA [ Homozygous mutant ]
The dog carries two copies of the mutant gene and
therefore it will pass the mutant gene to its entire offspring.
The dog is likely to develop Progressive Retinal Atrophy (prcd-PRA): (8094P / 8127) and will pass the mutant gene to its entire offspring
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. . |
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Price
for the above 2 tests
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£ 96.00 (including VAT)
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| To order:
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Download
Order Form from this link 
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Complete the order form and send it together
with your samples to the following address:
Laboklin (UK), 125 Northenden Road, Manchester, M33 3HF
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