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Australian Shepherd KC DNA bundle (MDR1+ prcd-PRA + CEA + HSF4)
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1 ) Collie Eye Anomaly (CEA) / Choroidal Hypoplasia (CH) *
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Re ISDS: Please note that this test is accepted by the ISDS provided that the sample is collected by a vet who should also sign a sample collection form which can be downloaded from the following link: ISDS DNA Bundle Order Form '
Kennel Club: results of this test is accepted by the Kennel Club |
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Breeds
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Australian Shepherd
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Australian Kelpie
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Bearded Collie
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Border collie
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Boykin Spaniel
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English shepherd
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Hokkaido
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Lancashire Heeler
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Longhaired Whippet
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Miniature American Shepherd
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Nova Scotia Duck tolling Retriever ( NSDTR )
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Rough Collie
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Shetland Sheepdog (Sheltie)
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Silken Windhound
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Smooth Collie
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Long Haired Whippet
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Kennel Club
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This test is part of the Official UK Kennel Club DNA Testing Scheme in Australian Shepherd , Bearded Collie , Border collie , Lancashire Heeler , Nova Scotia Duck tolling Retriever ( NSDTR ) , Rough Collie , Shetland Sheepdog (Sheltie) , and Smooth Collie.
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The Disease |
Collie Eye Anomaly is an inherited disease with recessive mode of inheritacne which results in abnormal development of the eye's choroid.The disease can be mild or servere, in the mild form of the disease, there is a thinning in the choroid layer of the eye but the dog's vision remains normal, however, dogs with the mild form of the disease can produce severly afected offspring.
In the Severe form of the disease, the dog can suffer serious loss of vision, Colobomas can be seen around and at the optic nerve head as outpouchings in the eye tissue layers. Colobomas may lead to secondary complications such as partial or complete retinal detachments and/or growth of new but abnormal blood vessels with bleeding inside the eye. The disease can affect one or both eyes and can lead to vision loss although this disease rarely lead to complete blindness.
* test performed by partner lab |
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Trait of Inheritance |
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Inheritance : AUTOSOMAL
RECESSIVE
trait
Sire
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Dam
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Offspring
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clear
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clear
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100% clear
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clear
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carrier
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50% clear + 50%
carriers
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clear
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affected
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100% carriers
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carrier
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clear
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50% clear + 50%
carriers
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carrier
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carrier
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25% clear + 25% affected
+ 50% carriers
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carrier
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affected
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50% carriers + 50%
affected
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affected
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clear
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100% carriers
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affected
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carrier
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50% carriers + 50%
affected
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affected
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affected
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100% affected
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Clear
Genotype: N / N [ Homozygous normal ]
The dog is noncarrier of the mutant gene.
It is very unlikely that the dog will develop Collie Eye Anomaly (CEA) / Choroidal Hypoplasia (CH) *. The dog will never pass the mutation to its offspring, and therefore it can be bred to any other dog.
Carrier
Genotype: N / CEA [ Heterozygous ]
The dog carries one copy of the mutant gene and one
copy of the normal gene.
It is very unlikely that the dog will develop Collie Eye Anomaly (CEA) / Choroidal Hypoplasia (CH) * but since it carries the mutant gene, it can pass it on to its offspring with the probability of 50%. Carriers should only be bred to clear dogs. Avoid breeding carrier to carrier because 25% of their offspring is expected to be affected (see table above)
Affected
Genotype: CEA / CEA [ Homozygous mutant ]
The dog carries two copies of the mutant gene and
therefore it will pass the mutant gene to its entire offspring.
The dog is likely to develop Collie Eye Anomaly (CEA) / Choroidal Hypoplasia (CH) * and will pass the mutant gene to its entire offspring
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. . |
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2 ) Hereditary Cataract (HSF4)
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Breeds
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Australian Shepherd
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Miniature American Shepherd
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Waeller (Wäller)
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Kennel Club
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This test is part of the Official UK Kennel Club DNA Testing Scheme in Australian Shepherd.
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The Disease |
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Cataracts are a leading form of blindness in the dog. The symptoms appear early in life and extend to complete blindness. The only effectice treatment is a surgical intervention. Homozygous affected dogs show more severe progress of the disease than heterozygous.
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. . |
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3 ) MDR1 Gene Varian / Ivermectin Sensitivity *
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Breeds
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American White Shepherd
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Australian Shepherd
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Bobtail
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Border collie
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Collie
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Elo
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English shepherd
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German Shepherd
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Longhaired Whippet
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McNab Shepherd (McNab Border Collie)
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Miniature American Shepherd
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Old English Sheepdog (Bobtail)
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Rough Collie
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Shetland Sheepdog (Sheltie)
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Silken Windhound
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Smooth Collie
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Waeller (Wäller)
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Kennel Club
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This test is part of the Official UK Kennel Club DNA Testing Scheme in Australian Shepherd , Border collie , Rough Collie , Shetland Sheepdog (Sheltie) , and Smooth Collie.
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The Disease |
MDR1 is a genetic disorder found in many dog breeds. Affected dogs, when treated with certain common drugs such as Ivermectin and loperamide (Imodium), are unable to pump out these drugs from the brain resulting in poisoning and neurologic symptoms ranging from tremors, anorexia and excess salivation to blindness, coma and even death. Some of these drugs such as Ivermectins, which vets prescribe extensively for the treatment of parasite infections, are able to cause toxicity at 1/200th of the dose required to cause toxicity in healthy dogs.
Scientists discovered that these dogs lack a protein (P-Glycoprotein), which is responsible for pumping out many drugs and toxins from the brain, and that affected dogs show signs of toxicity because they are unable to stop drugs from permeating their brains. Researchers have identified that this condition is due to a mutation in the multi-drug resistance gene [MDR1].
LIST OF DRUGS THAT CAUSE SENSITIVITY TO DOGS WITH MDR1 MUTATION
| Class A |
Do not use these drugs in dogs with MDR1 Gene Defect |
Ivermectine substances "Anti parasites": (Diapec®, Ecomectin®, Equimax®, Eqvalan®, Ivomec®, Noromectin®, Paramectin®, Qualimec®, Sumex®, Virbamec®)
Doramectine substances "Anti parasites": (Dectomax® )
Loperamide substances "ant diarrheal ":
(Imodium®)
Moxidectine substances "Anti Parasites" (Cydectin®, Equest®) |
Class B |
Use only under close control of veterinarian |
Cytostatics "Chemotherapy": (Vinblastine, Vincristine, Doxorubicine, Paclitaxel, Docetaxel, Methotrexat, Vincristine)
Immunosuppressive: (Cyclosporine A)
Heart glycosides: (Digoxine, Methyldigoxine)
Opioids: (Morphium)
Antiarrhythmics: (Verapamil, Diltiazem, Chinidine)
Antiemetics (Ondansetron, Domperidon, Metoclopramide )
Antibiotics (Sparfloxacin, Grepafloxacin, Erythromycin)
Antihistamin (Ebastin)
Glucocorticoid (Dexamethason)
Acepromazine (tranquilizer and pre-anesthetic agent) *
Butorphanol "analgesic and pre-anesthetic agent" *
Other drugs:
Etoposide, Mitoxantrone, Ondansetron, Paclitaxel, Rifampicin |
| Class C |
Can be used only in the permitted application form and dose! |
Selamectin (Stronghold®), Milbemax® and Advocate® . |
* In dogs with the MDR1 mutation, acepromazine and butorphanol tend to cause more profound and prolonged sedation in dogs . It is recommended to reduce the dose by 25% in dogs heterozygous for the MDR1 mutation (MDR1 / N) and by 30-50% in dogs homozygous for the MDR1 mutation (MDR1 / MDR1).
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Trait of Inheritance |
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Dogs that are homozygous for the mutation display, due to a non-functional transporter the ivermectin sensitive phenotype. They can show increased absorption of ivermectin and other substrates e.g. Digoxin, Vincristine, Doxorubicin, Cyclosporin A, Grepafloxacin, Dexamethasone and Loperamide (See list of drugs). Heterozygous animals (carriers) may show sensitivity to avermectins and other drugs. They are able to propagate the responsible mutation throughout the population and it is therefore important that carrier animals are detected prior to breeding.
Carriers mayhave sensitivity and should be treated with care
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Inheritance : AUTOSOMAL
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Description |
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This is a mutation-based gene test, which offers many advantages over other methods
The MDR1 gene variant can be detected, using molecular genetic testing techniques. By DNA testing the mutation can be shown directly. The testing is carried out by state of the art laboratory methods and therefore provides a very high accuracy. In general DNA tests can be done at any age. These tests identify both affected and carrier animals. The mutation can be shown directly, what clearly identifies homozygous affected animals. The genetic test offers the unique possibility to identify Ivermectin sensitive animals prior to treatment with Ivermectin and other drugs (see list). * partner lab
Please note drug list may not be up to date. The WSU Veterinary CLinical Pharmacology Lab may have a more updated list https://vcpl.vetmed.wsu.edu/problem-drugs. Please note that there maybe other problem drugs which may have not been yet identified.
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. . |
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4 ) Progressive Retinal Atrophy (prcd-PRA): (8094P / 8127)
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Kennel Club: results of this test is accepted by the Kennel Club
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Breeds
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American Cocker Spaniel
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American Eskimo
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Australian cattle dog
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Australian Shepherd
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Australian Stumpy Tail Cattle Dog
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Australian Stumpy tail cattle Dog
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Barbet (French Water Dog)
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Bearded Collie
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Bolognese
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Bolonka Zwetna
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Chesapeake Bay Retriever
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Chihuahua
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Chinese Crested
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Cockapoo (English)
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Cockapoo (American)
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Cocker Spaniel
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Dwarf poodle
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English Cocker Spaniel
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English shepherd
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Entlebuch Mountain dog
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Finnish Lapphund
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German Spitz
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Giant Schnauzer
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Golden Retriever
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Jack Russell Terrier
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Karelian Bear Dog
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Kuvasz
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Labradoodle
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Labrador Retriever
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Lagotto Romagnolo
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Lapponian Herder
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Markiesje
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Miniature Poodle
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Miniature American Shepherd
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Norwegian Elkhound
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Nova Scotia Duck tolling Retriever ( NSDTR )
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Parson Russell Terrier (PRT)
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Poodle
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Portuguese Waterdog
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Schipperke
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Australian Silky Terrier
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Spanish Water Dog
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Standard Poodle
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Swedish Lapp Hund
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Toy Poodle
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Waeller (Wäller)
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Yorkshire Terrier
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Kennel Club
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This test is part of the Official UK Kennel Club DNA Testing Scheme in American Cocker Spaniel , Australian cattle dog , Australian Shepherd , Barbet (French Water Dog) , Chesapeake Bay Retriever , Chinese Crested , Cocker Spaniel , English Cocker Spaniel , Entlebuch Mountain dog , Finnish Lapphund , Giant Schnauzer , Labrador Retriever , Miniature Poodle , Norwegian Elkhound , Nova Scotia Duck tolling Retriever ( NSDTR ) , Portuguese Waterdog , Spanish Water Dog , Standard Poodle , and Toy Poodle.
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The Disease |
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Progressive retinal atrophy (PRA) as an inherited disease occurs in many dog breeds and also in different forms. The form of progressive rod-cone degeneration (prcd-PRA) is a photoreceptor degeneration in dogs with varying ages of onset. This genetic disorder causes the degeneration of retinal cells in the eye: firstly, rod cells are affected, thus leading to progressive night blindness. Secondly, degeneration of the cone cells results in complete blindness of the dog, even in full light situations during the day.
Age of onset of clinical symptoms is typically in early adolescence or early adulthood. However, the onset of the disease may vary among different dog breeds.
Since diagnosis of retinal diseases in dogs may prove difficult, the genetic test on prcd-PRA helps to diagnose a specific disease and is also a useful tool for breeders to eliminate the mutated gene from the dog population.
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Trait of Inheritance |
The mutation in the PRCD gene which has been suggested to cause prcd-PRA has recently been published by the group of Gustavo D. Aguirre at the University of Pennsylvania, USA, and could be found in several dog breeds.
Prcd-PRA is inherited as an autosomal recessive trait. So there are three conditions a dog can be: it can be clear (genotype N/N or homozygous normal) meaning that it does not carry the mutation and will not develop the prcd-form of PRA. Since it also cannot pass the mutation onto its offspring, it can be mated to any other dog.
A dog which has one copy of the PRCD gene with the mutation and one copy without the mutation is called a carrier or heterozygous (genotype N/PRA); while it will not be affected by prcd-PRA, it can pass the mutation onto its offspring and should therefore only be mated to clear dogs.
Dogs that develop this form of PRA have two PRCD gene copies with the mutation (genotype PRA/PRA or homozygous affected); they will always pass the mutated gene onto their offspring and should also be mated only to clear dogs.. |
Inheritance : AUTOSOMAL
RECESSIVE
trait
Sire
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Dam
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Offspring
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clear
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clear
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100% clear
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clear
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carrier
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50% clear + 50%
carriers
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clear
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affected
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100% carriers
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carrier
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clear
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50% clear + 50%
carriers
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carrier
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carrier
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25% clear + 25% affected
+ 50% carriers
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carrier
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affected
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50% carriers + 50%
affected
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affected
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clear
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100% carriers
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affected
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carrier
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50% carriers + 50%
affected
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affected
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affected
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100% affected
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Clear
Genotype: N / N [ Homozygous normal ]
The dog is noncarrier of the mutant gene.
It is very unlikely that the dog will develop Progressive Retinal Atrophy (prcd-PRA): (8094P / 8127). The dog will never pass the mutation to its offspring, and therefore it can be bred to any other dog.
Carrier
Genotype: N / PRA [ Heterozygous ]
The dog carries one copy of the mutant gene and one
copy of the normal gene.
It is very unlikely that the dog will develop Progressive Retinal Atrophy (prcd-PRA): (8094P / 8127) but since it carries the mutant gene, it can pass it on to its offspring with the probability of 50%. Carriers should only be bred to clear dogs. Avoid breeding carrier to carrier because 25% of their offspring is expected to be affected (see table above)
Affected
Genotype: PRA / PRA [ Homozygous mutant ]
The dog carries two copies of the mutant gene and
therefore it will pass the mutant gene to its entire offspring.
The dog is likely to develop Progressive Retinal Atrophy (prcd-PRA): (8094P / 8127) and will pass the mutant gene to its entire offspring
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. . |
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Price
for the above 4 tests
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£ 138.00 (including VAT)
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| To order:
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Download
Order Form from this link 
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Complete the order form and send it together
with your samples to the following address:
Laboklin (UK), 125 Northenden Road, Manchester, M33 3HF
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