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**NEW**



Bengal Special offer:
4 Bengal Specific DNA tests for just £72.00 incl VAT
Bengal DNA bundle (rdAc-PRA + b-PRA + PK-Def + Blood Groups) 



British Special offer:
4 Breed Specific DNA tests for just £72.00 incl VAT
British Short / Long Hair DNA bundle (PKD + pd-PRA + ALS + Blood Groups)



Burmese Special offer:
4 Breed Specific DNA tests for just £72.00 incl VAT
Burmese DNA bundle (Hypokalemia (BHK) + Head Defect + Gangliosidosis (GM2) + Blood Groups



Birman Special offer:
5 Breed Specific DNA tests for just £72.00 incl VAT
Birma DNA bundle (PKD + pd-PRA + Hypotrichiose + MPS6 + Blood Groups)



Maine Coon Special offer:
5 Breed Specific DNA tests for just £72.00 incl VAT
Maine Coon DNA bundle (HCM1 + SMA + PK-Def + F11 + Blood Groups)



Ragdoll Special offer:
5 Breed Specific DNA tests for just £72.00 incl VAT
Ragdoll DNA bundle (HCM1 + HCM3 + PKD + pd-PRA + Blood Groups)



Norwegian Special offer:
4 Breed Specific DNA tests for just £72.00 incl VAT
Norwegian Forest DNA bundle (PK-Def + Amber + GSD4 + Blood Groups)



Feline Special Offer:
8 cat DNA tests for just £84.95 including VAT
HCM, HCR, GSD4, PKD, PRA, PK-Def., SMA, Blood Groups

new bundle: White Swiss Shepherd DNA bundle
new test:      Limb Girdle Muscular Dystrophy (LGMD) in Dachhsund  
new test:      Dyserythropoietic Anemia and Myopathy Syndrome (DAMS) in English Springer Spaniel
new Kennel Club DNA testing schemes with LABOKLIN:
   Dyserythropoietic Anemia and Myopathy Syndrome (DAMS) in English Springer Spaniel
  Primary Open Angle Glaucoma (POAG) in Basset Hound, Basset Fauve de Bretagne (Petit), and Norwegian Elkhound


BRAF Mutation ( transitional cell carcinoma )

Test number: 8675
Gene: BRAF
Price: £ 108.24 (including VAT)
Breed
All Dog Breeds .
Description

Droplet Digital PCR

Transitional cell carcinomas (TCC) are the most common malignant tumors of the canine urinary tract. Mochizuki et al. (PLoS ONE 2015a, 10(6):e0129534; PLoS ONE 2015b, 10(12):e0144170) identified the BRAF variant V595E in approximately 85% of canine TCC cases by DNA sequencing of TCC tumor cells as well as in prostate carcinoma. This BRAF variant V595E analysis is a non-invasive and simple tool used to confirm the diagnosis in cases where TCC is suspected. However, it is important to note that only a positive identification of the mutation is valuable, since the variant could never be demonstrated in animals with cystitis, polyp or other urinary bladder neoplasms. The absence of the BRAF mutation may be due to the fact that the tumor is not caused by a BRAF mutation or that the submitted material did not contain mutated cells. In addition, the tumor might not be a TCC, but a tumor of other origin, a polyp or cystitis.

Detection of BRAF variant V595E.


BRAF diagnosis by droplet digital PCR
('Affected events': Detection of cells with BRAF mutation;
'free events': Detection of cells without BRAF mutation)

  • Indications:
    • It was not possible to get a reliable histological/cytological diagnosis.
    • Screening for transitional cell carcinoma in certain predisposed terrier breeds (e.g. Scottish Terrier, Fox Terrier, Jack Russell Terrier, West Highland White Terrier)
    • Difficult patient
  • Only a positive result is conclusive.
  • Reasons for negative results:
    • The transitional cell/prostate carcinoma is not caused by BRAF mutation (approx. 30 – 50% of these carcinomas depending on the breed).
    • There were no mutated cells present in the sample.
    • It is not a transitional cell/prostate carcinoma.
  • The test cannot be used for cats.

 
Further reading
Identification of BRAF Variant V595E in urine, smears and paraffin-embedded tissuePDF file
BRAF variant V595E in urinehtml file
Sample Requirements
5 - 10 ml urine, formalin fixed tissue, or smears

If urinary bladder/urethral carcinoma (transitional cell carcinoma) is suspected:

  • Urinary sediment (especially early morning urine; fluid + smear)
  • Aspiration cytology (smears)
  • Biopsies (formalin-fixed)
If prostate carcinoma is suspected:
  • Smears rich in cells or biopsies (formalin-fixed)
Turnaround
1-3 weeks
Price
£ 108.24 (including VAT)

To order:




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