|
Welsh Corgi DNA bundle (Brachyuria + IVDD-risk + DM exon2 + rcd3-PRA + vWD1)
|
|
|
|
|
|
1 ) Brachyury (Bobtail Gene / Short Tail)
|
|
Breeds
|
|
Australian Shepherd
,
Australian Stumpy tail cattle Dog
,
Austrian Pinscher
,
Bourbonnais Pointer
,
Bouvier des Ardennes
,
Brazilian Terrier
,
Brittany Spaniel
,
Croatian Sheepdog
,
Danish Farm Dog
,
Jack Russell Terrier
,
Karelian Bear Dog
,
Miniature American Shepherd
,
Mudi
,
Polish Lowland sheepdog
,
Pyrenean Shepherd
,
Savoy Sheepdog
,
Schipperke
,
Spanish Water Dog
,
Spanish Waterdog
,
Swedish Farm Dog
,
Swedish Vallhund
,
Welsh Corgi
.
|
|
|
|
Description |
|
this test enables you to identify if a dog is docked or has a natural short tail. This conditioned is caused by the Brachyury mutation, the trait of inheritance is autosomal dominant which means that one copy of the mutation is enough to cause the natural short tail. Dogs with two copies of the normal gene will have normal tail. No dogs were found to have two copies of the mutation because it is thought that embryos with two copies of the mutation T/T are either die in the womb and reabsorbed by the bitch resulting in smaller litter size, or they die shortly after birth.
Please note that the Short tail phenotype includes either a complete lack of vertebrae or a short tail with variable length. The test does not differentiate between tailless and stumpy tail
Possible results:
Normal (N/N): The dog has two copies of the normal gene and will have a normal tail. Heterozygous (N/T): The dog carries one copy of the normal gene and one copy of the mutant gene; the dog has a natural short tail. Heterozygous dogs can pass the mutation to their offspring with a probability of 50%.
Homozygous for the mutation (T/T): does not exist and thought to be lethal.
Research found that there is no association between the bobtail mutation and the short tail phenotype in the following breeds: Boston Terrier, English Bulldog, King Charles Spaniel, Miniature Schnauzer, Parson Russell Terrier, Rottweiler.
|
|
|
|
| |
|
|
Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. . |
|
|
| |
|
2 ) Chondrodystrophy (CDDY with IVDD Risk) and Chondrodysplasia (CDPA)
|
|
Breeds
|
|
All Dog Breeds
,
American Cocker Spaniel
,
Basset
,
Beagle
,
Bichon Frise
,
Cavalier King Charles Spaniel
,
Cardigan Welsh Corgi
,
Chesapeake Bay Retriever
,
Chihuahua
,
Coton de Tulear
,
Dachshund
,
Dandie Dinmont Terrier
,
English Springer Spaniel
,
French Bull Dog
,
Havanese - Bichon Havanese
,
Jack Russell Terrier
,
Miniature Poodle
,
Miniature Wire haired Dachshund
,
Miniature Long Haired Dachshund
,
Miniature Smooth Haired Dachshund
,
Nova Scotia Duck tolling Retriever ( NSDTR )
,
Pekingese
,
Pembroke Welsh Corgi
,
Portuguese Waterdog
,
Scottish Terrier
,
Shih Tzu
,
Toy Poodle
,
West Highland White Terrier
,
Welsh Corgi
.
|
|
|
|
Description |
 |
 |
|
Chondrodystrophy (CDDY with IVDD Risk) and Chondrodysplasia (CDPA)
The test checks for two mutations: CDDY with IVDD Risk, and CDPA.
Chondrodystrophy CDDY (FGF4-12) which causes short legs and the risk of developing Intervertebral Disc Disease (IVDD).
Chondrodysplasia CDPA (FGF4-18), which causes the short legged phenotype in a number of breeds.
Chondrodystrophy (CDDY with IVDD Risk) is a trait that is common to many dog breeds and it is characterised by shorter legs due to shorter long bones. CDDY can also be associated with Intervertebral Disc Disease (IVDD) due to premature degeneration of the intervertbral disc. The intervertebral disc lie between the vertebrae and it is made of a cartilage which separate vertebrae from each other, absorb shocks and allow slight movement of the vertebrae. In affected dogs, premature calcification of part of the disc at early age (from birth to 1 year of age) results in degeneration of all discs in young dogs. These abnormal discs are susceptible to herniation into the spinal canal where the inflammation, and hemorrhage can cause severe pain and neurological dysfunction. CDDY is inherited as a semi-dominant trait which means that dogs with 2 copies of the mutation are smaller than dogs with only 1 copy. As for IVDD, the inheritance follows a dominant mode, meaning that 1 copy of CDDY mutation is sufficient to predispose dogs to IVDD.
The CDDY mutation has been found in breeds such as: Basset Hound, Beagle, Bichon Frise, Cardigan Welsh Corgi, Cavalier King Charles Spaniel, Chesapeake Bay Retriever, Chihuahua, American Cocker Spaniel, Coton de Tulear, Dachshund, Dandie Dinmont Terrier, English Springer Spaniel, French Bulldog, Havanese, Jack Russell Terrier, Nova Scotia Duck Tolling Retriever, Pekingese, Pembroke Welsh Corgi, Poodle (Miniature and Toy), Portuguese Water Dog, Scottish Terrier, Shih Tzu.
The second mutation CDPA explains the short-legged phenotype known as chondrodysplasia (CDPA) in breeds such as Basset Hound, Pembroke Welsh Corgi, Dachshunds, West Highland White Terriers and Scottish Terriers. CDPA inheritance is considered to follow am autosomal dominant mode.
In some breeds both mutations are present and so breeders will be able to plan breeding to reduce occurrence of CDDY, while retaining the short-legged phenotype CDPA.
|
|
|
|
| |
|
|
Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. . |
|
|
| |
|
3 ) Degenerative Myelopathy / Degenerative Radiculomyelopathy) DM (Exon 2)
|
|
Breeds
|
|
Airedale Terrier
,
Alaskan Malamute
,
All Dog Breeds
,
American Eskimo
,
Bernese Mountain Dog
,
Bloodhound
,
Borzoi (Russian Wolfhound)
,
Boxer
,
British Timber Dog
,
Cavalier King Charles Spaniel
,
Canaan Dog
,
Cardigan Welsh Corgi
,
Chesapeake Bay Retriever
,
Fox Terrier
,
French Bull Dog
,
German Shepherd
,
Glen Of Imaal Terrier ( GIT )
,
Golden Retriever
,
Pyrenean Mountain Dog (Great Pyrenees)
,
Hovawart
,
Pumi ( Hungarian Pumi / Pumik )
,
Jack Russell Terrier
,
Kerry Blue Terrier
,
Labrador Retriever
,
Lakeland Terrier
,
Northern Inuit
,
Nova Scotia Duck tolling Retriever ( NSDTR )
,
Pembroke Welsh Corgi
,
Poodle
,
Pug
,
Rhodesian Ridgeback
,
Rough Collie
,
Soft Coated Wheaten Terrier
,
Shetland Sheepdog (Sheltie)
,
Smooth Collie
,
Utonagan
,
Wire Fox Terrier
.
|
|
|
|
Kennel Club
|
|
This test is part of the Official UK Kennel Club DNA Testing Scheme in Chesapeake Bay Retriever , French Bull Dog , German Shepherd , Nova Scotia Duck tolling Retriever ( NSDTR ) , Rough Collie , and Smooth Collie.
|
|
|
|
The Disease |
|
Canine degenerative myelopathy (also known as chronic degenerative radiculomyelopathy) is a progressive disease of the spinal cord in older dogs. The disease has an insidious onset typically between 7 and 14 years of age. It begins with a loss of coordination (ataxia) in the hind limbs. As of July 15, 2008 the mutated gene responsible for DM has been found present in 43 breeds including German Shepherds, Boxers, Chesapeake Bay Retrievers, Rhodesian Ridgebacks, and both breeds of Welsh Corgis. The disease is chronic and progressive, and resulting in paralysis.
|
|
|
|
|
Clinical Signs |
|
Degenerative myelopathy initially affects the back legs and causes muscle weakness and loss, and lack of coordination. These cause a staggering effect that may appear to be arthritis. The dog may drag one or both rear paws when it walks. This dragging can cause the nails of one foot to be worn down. The condition may lead to extensive paralysis of the back legs. As the disease progresses, the animal may display symptoms such as incontinence and has considerable difficulties with both balance and walking. If allowed to progress, the animal will show front limb involvement and extensive muscle atrophy. Eventually cranial nerve or respiratory muscle involvement necessitates euthanasia.
Progression of the disease is generally slow but highly variable. The animal could be crippled within a few months, or may survive up to three years
|
|
|
|
Trait of Inheritance |
|
Tow alleles are invloved in Degenerative Myelopathy, A and G, therefore a test result can be A/A, A/G, or G/G.
Mode of inheritance is autosomal recessive with variable penetrance;
|
Inheritance : AUTOSOMAL
RECESSIVE
trait
Sire
|
|
Dam
|
|
Offspring
|
| |
|
|
|
|
|
clear
|
 |
clear
|
 |
100% clear
|
| |
|
|
|
|
|
clear
|
|
carrier
|
|
50% clear + 50%
carriers
|
| |
|
|
|
|
|
clear
|
|
affected
|
|
100% carriers
|
| |
|
|
|
|
|
carrier
|
|
clear
|
|
50% clear + 50%
carriers
|
| |
|
|
|
|
|
carrier
|
|
carrier
|
|
25% clear + 25% affected
+ 50% carriers
|
| |
|
|
|
|
|
carrier
|
|
affected
|
|
50% carriers + 50%
affected
|
| |
|
|
|
|
|
affected
|
|
clear
|
|
100% carriers
|
| |
|
|
|
|
|
affected
|
|
carrier
|
|
50% carriers + 50%
affected
|
| |
|
|
|
|
|
affected
|
|
affected
|
|
100% affected
|
Clear
Genotype: N / N [ Homozygous normal ]
The dog is noncarrier of the mutant gene.
It is very unlikely that the dog will show signs of the Degenerative Myelopathy
Carrier
Genotype: N / DM (Exon 2) [ Heterozygous ]
The dog carries one copy of the mutant gene and one
copy of the normal gene.
It is very unlikely that the dog will show signs of the Degenerative Myelopathy
Affected
Genotype: DM (Exon 2) / DM (Exon 2) [ Homozygous mutant ]
The dog carries two copies of the mutant gene and
therefore it will pass the mutant gene to its entire offspring.
The dog may or may not show signs of the disease
|
|
|
| |
|
Sample Requirements |
Buccal swabs or 0.5 - 1 ml blood in EDTA Blood Tube . |
|
|
| |
|
4 ) Progressive Retinal Atrophy (rcd3 PRA)
|
|
Breeds
|
|
Cardigan Welsh Corgi
,
Chinese Crested
,
Pomeranian
.
|
|
|
|
Kennel Club
|
|
This test is part of the Official UK Kennel Club DNA Testing Scheme in Cardigan Welsh Corgi.
|
|
|
|
The Disease |
|
Progressive retinal atrophy (PRA) is a leading hereditary cause of blindness in pedigree dogs as is its counterpart retinitis pigmentosa (RP) in humans. PRA shows genetic heterogeneity, as does RP, with several distinct forms already recognized and several more remaining to be investigated.
Pups show signs of night-blindness by 6 weeks of age. By the age of 1-2 years most affected dogs are completely blind.
|
|
|
|
|
Trait of Inheritance |
|
rcd3 PRA follows an autosomal recessive trait of inheritance.
Since vision loss might be recognised first when the dog is several years old, it is important to determine the actual status of the dog before breeding it.
|
Inheritance : AUTOSOMAL
RECESSIVE
trait
Sire
|
|
Dam
|
|
Offspring
|
| |
|
|
|
|
|
clear
|
|
clear
|
|
100% clear
|
| |
|
|
|
|
|
clear
|
|
carrier
|
|
50% clear + 50%
carriers
|
| |
|
|
|
|
|
clear
|
|
affected
|
|
100% carriers
|
| |
|
|
|
|
|
carrier
|
|
clear
|
|
50% clear + 50%
carriers
|
| |
|
|
|
|
|
carrier
|
|
carrier
|
|
25% clear + 25% affected
+ 50% carriers
|
| |
|
|
|
|
|
carrier
|
|
affected
|
|
50% carriers + 50%
affected
|
| |
|
|
|
|
|
affected
|
|
clear
|
|
100% carriers
|
| |
|
|
|
|
|
affected
|
|
carrier
|
|
50% carriers + 50%
affected
|
| |
|
|
|
|
|
affected
|
|
affected
|
|
100% affected
|
Clear
Genotype: N / N [ Homozygous normal ]
The dog is noncarrier of the mutant gene.
It is very unlikely that the dog will develop Progressive Retinal Atrophy (rcd3 PRA). The dog will never pass the mutation to its offspring, and therefore it can be bred to any other dog.
Carrier
Genotype: N / PRA [ Heterozygous ]
The dog carries one copy of the mutant gene and one
copy of the normal gene.
It is very unlikely that the dog will develop Progressive Retinal Atrophy (rcd3 PRA) but since it carries the mutant gene, it can pass it on to its offspring with the probability of 50%. Carriers should only be bred to clear dogs. Avoid breeding carrier to carrier because 25% of their offspring is expected to be affected (see table above)
Affected
Genotype: PRA / PRA [ Homozygous mutant ]
The dog carries two copies of the mutant gene and
therefore it will pass the mutant gene to its entire offspring.
The dog is likely to develop Progressive Retinal Atrophy (rcd3 PRA) and will pass the mutant gene to its entire offspring
|
|
|
|
Description |
|
Progress in molecular genetics has allowed the identification of the gene mutation responsible for PRA.
By DNA testing, the responsible mutation can be shown directly. This method provides a very high accuracy test and can be done at any age. It offers the possibility to distinguish between affected and clear dogs. This is an essential information for controlling the disease in the breed.
|
|
|
|
|
Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. . |
|
|
| |
|
5 ) von Willebrand disease Type I (vWD I)
|
|
Breeds
|
|
Bernese Mountain Dog
,
Cockapoo (English)
,
Cockapoo (American)
,
Coton de Tulear
,
Doberman
,
Drentsche Patrijschond
,
German Pinscher
,
Irish Red and White Setter
,
Irish Setter
,
Kerry Blue Terrier
,
Kromfohrländer
,
Labradoodle
,
Manchester Terrier
,
Miniature Poodle
,
Papillon
,
Pembroke Welsh Corgi
,
Poodle
,
Stabyhound ( Stabijhoun )
,
Standard Poodle
,
Toy Poodle
.
|
|
|
|
Kennel Club
|
|
This test is part of the Official UK Kennel Club DNA Testing Scheme in Doberman , Manchester Terrier , Papillon , and Standard Poodle.
|
|
|
|
The Disease |
We are pleased to announce that Laboklin obtained an exclusive European License to perform this important genetic tes from Vet Gen LCC the owner of the European patentt.
Von Willebrand disease (vWD) is probably the most common inherited bleeding disorder in dogs. It is caused by lack of von Willebrand factor which is a protein that plays a key role in the blood clotting process resulting in prolonged bleeding. The disorder occurs in varying degrees of severity ranging from trivial bleeding to excessive life threatening haemorrhages. |
|
|
|
|
Clinical Signs |
Symptoms include spontaneous bleeding from the nose, gum and other mucous membranes. Excessive bleeding occurs after an injury, trauma or a surgery. Often dogs don’t show clinical signs until something starts the bleeding, such as nail trimming, teething, spaying, sterilizing, tail docking, cropping or other causes. Bleeding also occurs internally in the stomach, intestines, urinary tracts, the genitals and / or into the joints.
Type I von Willebrand's disease is considered relatively mild when compared to Type II in Scotch Terriers and Shetland Sheep Dogs and Type III in the German Wirehaired pointer, Type II and Type III are much more severe than type I. |
|
|
|
Trait of Inheritance |
|
vWD Type I is transmitted as autosomal incomplete dominant trait . This means that a dog that is genetically clear (also called homozygous normal) will not develop the vWD disorder and will not pass it to its offspring. Carrier dogs which carries one copy of the abnormal gene and another normal copy (also known as heterozygous) will have bleeding tendency. These carrier dogs will pass the abnormal gene to their offspring with a probability of 50%, the trait is called incomplete dominance because carrier dogs may not develop the disorder at all but they will still pass the abnormal gene to their offspring. Because it is very uncommon for carriers to show symptoms of vWD this condition is treated as Recessive. Affected dogs (carry two copies of the abnormal gene) will develop the vWD disorder and will pass the abnormal gene to each of their offspring
|
Inheritance : AUTOSOMAL
RECESSIVE
trait
Sire
|
|
Dam
|
|
Offspring
|
| |
|
|
|
|
|
clear
|
|
clear
|
|
100% clear
|
| |
|
|
|
|
|
clear
|
|
carrier
|
|
50% clear + 50%
carriers
|
| |
|
|
|
|
|
clear
|
|
affected
|
|
100% carriers
|
| |
|
|
|
|
|
carrier
|
|
clear
|
|
50% clear + 50%
carriers
|
| |
|
|
|
|
|
carrier
|
|
carrier
|
|
25% clear + 25% affected
+ 50% carriers
|
| |
|
|
|
|
|
carrier
|
|
affected
|
|
50% carriers + 50%
affected
|
| |
|
|
|
|
|
affected
|
|
clear
|
|
100% carriers
|
| |
|
|
|
|
|
affected
|
|
carrier
|
|
50% carriers + 50%
affected
|
| |
|
|
|
|
|
affected
|
|
affected
|
|
100% affected
|
Clear
Genotype: N / N [ Homozygous normal ]
The dog is noncarrier of the mutant gene.
It is very unlikely that the dog will develop von Willebrand disease Type I (vWD I). The dog will never pass the mutation to its offspring, and therefore it can be bred to any other dog.
Carrier
Genotype: N / vWD [ Heterozygous ]
The dog carries one copy of the mutant gene and one
copy of the normal gene.
The dog is very unlikely to develop von Willebrand disease Type I (vWD I) and if it does it will be in a mild form. Since it carries the mutant gene, it can pass it on to its offspring with the probability of 50%.
Affected
Genotype: vWD / vWD [ Homozygous mutant ]
The dog carries two copies of the mutant gene and
therefore it will pass the mutant gene to its entire offspring.
The dog is likely to develop von Willebrand disease Type I (vWD I) and will pass the mutant gene to its entire offspring
|
|
|
|
Description |
|
The Mutation-based Test and its Advantages
A new DNA test has now been developed for the type I vWD.
Genetic testing makes it possible to identify whether a dog is clear, carrier or affected. This is vital to eliminate this condition from the breed within 2-3 generations.
The new DNA test can identify the responsible mutation directly.
This DNA test can be done at any age and unambiguously classifies dogs into affected, carriers and clear. The test enables breeders to eliminate the vWD disease gene from the Poodles. Carriers can be clinically normal because of a low penetrance or expressivity of the disease. This information is essential for controlling this disorder in the breed.
Breeders and owners should view vWD as a significant health risk and strive to get rid of the mutated gene. The discovery of the mutation, and the recent development of a DNA test, now provides just that opportunity.
|
|
|
|
|
Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. . |
|
|
| |
|
Price
for the above 5 tests
|
|
£ 156.00 (including VAT)
|
|
|
 |
| To order:
-
Download
Order Form from this link 
-
Complete the order form and send it together
with your samples to the following address:
Laboklin (UK), 125 Northenden Road, Manchester, M33 3HF
|
|
|
|
|
