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Coat Colour DNA bundle: Loci A + B + D1 + E1 + I + K + S
Test number: 8654 Price: £ 156.00 (including VAT) for all 7 tests
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1 ) Coat Colours: A-Locus Agouti ( fawn, sable, black and tan/tricolor, recessive black)
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Description |
The A locus is responsible for a number of common coat patterns in the dog. Expression of all of them requires any combination of two Ky or Kbr alleles at the K locus, and at
least one E or EM allele at the E locus. The gene involved is the Agouti gene,
and variations in it are responsible for fawn and sable dogs (Ay),
wild type (aw), tan points (at), and recessive black(a).
analysis proves absence or presence of the mutation typically responsible for fawn or sable. In fawn/ sable dogs this test shows if other agouti alleles are present
but hidden (only one copy of Ay). It also demonstrates how many
copies of this allele are hidden in dogs, which cannot express agouti types (KBKB, KB kbr, KB ky,
at the k locus and/or ee at the E locus).
shows whether a black dog is black due to “recessive black,” or the more common black
at the K locus. It also reveals whether a non-black animal carries “recessive
black.” (e.g. German Shepherd Dog, Shetland Sheepdog)
at allele (tan points, tricolors)
There is no direct test yet for this recessive allele (, but in some breeds, carriers may be deduced by use of the other two agouti tests.
In breeds where only the A y and a t alleles
are present, the A y test can be used to see if the fawn/sable dog is A y/A y (homozygous)
or only has one A y (heterozygous).
If it only has one, the other allele must be a t.
(e.g. Afghans,
Collies, Cardigan Welsh Corgi, Dachshund, Norwich Terrier, Staffordshire
Terrier) In breeds where
only A y, a t and
“a” alleles are present, both the A y test
and the “a” test need to be performed. Any alleles unaccounted for by these two
tests will be a t. For example, if a dog is A y/A y both
alleles are accounted for. If a fawn/sable dog only has a single A y,
then the other allele must either be an “a” or an a t, and this can be
determined by running the recessive black (“a”) test. Examples of breeds:< Shetland
Sheepdog, Belgians (Malinois, Groenendael).
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. .
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2 ) Coat Colour: B Locus (bd, bc, bs) Brown Coat Colour
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Breeds
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All Dog Breeds
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American Cocker Spaniel
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Australian Shepherd
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Bedlington Terrier
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Border Collie
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Welsh Corgi (Cardigan)
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Dachshund
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Dalmatian
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Doberman Pinscher
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English Cocker Spaniel
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Flatcoated Retriever
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Fox Terrier
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French Bull Dog
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Galgo Espanol
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German Longhaired Pointer
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German Shorthair Pointer
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Gordon Setter
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Griffon Bruxellois
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Irish Soft Coated Wheaten Terrier
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Koolie ( Australian Koolie )
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Labrador Retriever
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Miniature Pinscher
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Newfoundland
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Pointer
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Portuguese Waterdog
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Scottish Terrier
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Weimaraner
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Description |
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This test is for the 'bd', 'bc' and 'bs'variants, which are described in all dog breeds and are responsible for the brown coat colour, which is also known in some breeds as liver, chocolate, chestnut, sedge, and less frequently, red. Two copies of the b-allele are needed to dilute black pigment to brown. For red or yellow dogs, the brown allele does not dilute the hair colour, but will change the colour of nose and foot pads from black to brown if two brown alleles are present.
When one of the variants is found homozygous (bd/bd, bc/bc or bs/bs), dark pigment (eumelanin) is diluted to brown in the pigmented areas. However, when several variants of the B-locus are found in heterozygous state (example N/bd and N/bc), it is not always possible to directly determine the influence on the eumelanin because this depends on whether the variants are located on the same or different chromosmes, however, the dog will definitely pass the variants to its offspring.
Australian Shepherd and Lancashire Heeler Please note there are two additional rare variants, which are not analysed in this test. The 'b4' variant which is only found in few Australian Shephered lines, and the 'be' variant which is only found in Lancashire Heeler, and therefore for a complete analysis in Australian Shepherd and Lancashire Heeler, you need to order this test in addition to the rare variant test . You can take advantage of our special offer 'second coat colour test at half price (excluding bundles)'.
French Bulldog
In French Bulldog, in addition to the B-locus, the Cocoa gene is also responsible for the brown coat colour, and therefore, in addition to this test you also need to order the Cocoa coat colour test . You can of course take advantage of our special offer 'second coat colour test at half price (excluding bundles)'.
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. .
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3 ) Coat Colours: D-Locus D1 ( Dilution )
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Breeds
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All Dog Breeds
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Border Collie
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Boston Terrier
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Bulldog (English)
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Chihuahua
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Doberman Pinscher
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French Bull Dog
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German Pinscher
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Koolie ( Australian Koolie )
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Labrador Retriever
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Large Munsterlander
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Miniature Pinscher
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Newfoundland
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Rhodesian Ridgeback
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Staffordshire Bull Terrier
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Description |
The D locus is the primary locus associated with diluted pigment, which results in coats that would otherwise be black or brown instead showing up as gray, or blue in the case of black, and pale brown or Isabella in the case of brown. The melanophilin gene has recently been shown to be responsible, but not all of the dilute causing mutations have been identified yet.
A recessive mutation in the melanophilin gene was identified as the cause of colour dilution phenotypes in the dog. Two alleles (variants) are described: the dominant full colour (D) and the recessive dilute (d). Two copies of dilute are needed to lighten black pigment to grey (often called blue) and red pigment to cream (also called buff). A diagnostic DNA test identifies the specific variants of the MLPH gene.
Please note that in the Chow Chow, Thai Ridgeback and Sloughi breeds, there is another mutation that can cause coat colour dilution, it is the D2 Locus mutation and in those breeds both D1 Locus and D2 Locus mutations must be tested for complete analysis.
Please note that in the Chihuahua, Italian Greyhound and Hungarian Pumi breeds, there is another mutation that can cause coat colour dilution, it is the D3 Locus mutation and in those breeds both D 1Locus and D3 Locus mutations must be tested for complete analysis.
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. .
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4 ) Coat Colours: E-Locus E1 (yellow, lemon, red, cream and appricot)
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Breeds
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Afghan Hound
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All Dog Breeds
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Australian Cattle Dog
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Australian Shepherd
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Beagle
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Border Collie
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Brittany ( Brittany Spaniel )
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Welsh Corgi (Cardigan)
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Chinese Chow Chow
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Cocker Spaniel
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Dachshund
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Dalmatian
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Doberman Pinscher
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English Cocker Spaniel
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English Setter
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English Springer Spaniel
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Field Spaniel
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Flatcoated Retriever
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Foxhound
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French Bull Dog
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German Longhaired Pointer
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German Shepherd
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German Wirehaired Pointer
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Gordon Setter
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Koolie ( Australian Koolie )
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Labrador Retriever
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Lowchen
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Miniature Schnauzer
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Pointer
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Pomeranian
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Poodle
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Pudelpointer
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Description |
Coat Colours
As with other mammals, dogs have two major types of pigment in their coat: dark pigment (Eumelanin) which is responsible for black and brown in the pigmented areas, and yellow pigment (Phaeomelanin) which is responsible for red, yellow, orange, golden, cream, apricot in the pigmented areas. The colour varieties seen in dogs are due to genes controlling the amount, extent, and distribution of these two colour pigments.
E-Locus
The E-Locus has a number variants, the 'e1' variant which is responsible for the yellow colours in most breeds, the rare 'e' variants which are repsonsible for the same but in certain breeds, and the special variants 'eA', 'eg' and 'eh' which are responsible for specific colours, some of them occurs only in specific breeds including domino, grizzle, sable and hare-pied. The EM variant is also located at the E-Locus and esponsible for the melanistic mask. This test is only for 'e1' variant.
The 'e1' variant
This test detects the 'e1' variant of the E-Locus, the 'e1' variant is responsible for the red, yellow, orange, golden, cream, apricot coat in the pigmented area in most breeds. The 'e1' variant is recessive which means that two copies of 'e1' (e1/e1) are needed for the dog to be red, yellow, orange, golden, cream, apricot in the pigmented areas. This test number 8018 which is offered in this test as an individual test, however, it is also included in our popular Laboklin Coat Colour Bundle (bundle number 8654)
Australian Cattle Dog
Please note there are additional rare E-Locus variants, which are not analysed in this test. The 'e2' variant is found in Australian Cattle Dog only and therefore for a complete analysis in Australian Cattle Dog, you need to order this test 'e1' in addition to the rare variant test . You can take advantage of our special offer additional coat colour test at half price'.
E-Locus Special colours: 'eA', 'eG' and 'eH'
Testing for these special colours is avaialable in a separate test, for more information check test number 8682 special E-Locus variants 'eA', 'eG' and 'eH'. You can take advantage of our special offer additional coat colour test at half price'.
EM-Locus Melanistic Mask
Testing for Melanistic Mask is available in a separate test, for more information check test number 8146 EM-Locus (Melanistic Mask). You can take advantage of our special offer additional coat colour test at half price'.
Order of dominance: EM> EG> E> EH> e.
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. .
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5 ) Coat Colours: K- Locus
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Please note that this test will only check for the KB and ky alleles, it does not check for the kbr (brindle). Laboklin no longer offers a test for Brindle.
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Description |
The K locus plays a pivotal role in coat colour. This locus is a relative newcomer in our
understanding of canine colour, and includes traits formerly attributed by some
to other genes.
The dominant allele in the series is KB, which is responsible for self-colouring,
or solid coloured fur in pigmented areas. This trait was formerly attributed to
the Agouti (A) locus as AS, but recent breeding studies had shown
this not to be the case.
There are two other alleles, kbr, and ky. KB is
dominant to both kbr and ky,
while kbr is dominant only
to ky. kbr is
responsible for the brindle. The recessive allele, ky, allows the
basic patterns of the A locus to be expressed. So too does the kbr allele,
but with brindling of any tan, fawn, or tawny areas. Any animal with at least
one KB allele will be
self-coloured.
Any animal with
at least one kbr allele,
and no KB alleles will be
brindled on agouti background (see A locus). Any animal with two ky alleles
will show agouti patterns (see A locus).
and
ky
LABOKLIN can presently test for these two alleles. In some breeds, where no
brindle is present, this represents a complete analysis of the locus. An example
would be the Pug. In breeds where the breed standard disqualifies all but self-colored
dogs, testing for these two alleles is once again all that is needed. Any animal
with two KB alleles cannot
produce anything except self-coloured offspring. A prime example here is the
Labrador retriever. In breeds where many variations are allowed, these tests can
help predict the probability of potential litters to include fawn, sable, tawny,
tan point, tricolor or recessive black puppies.
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. .
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6 ) Coat Colour: S-locus (piebald, spotted white)*
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Trait of Inheritance |
White spotting in dogs is mostly caused by variations of MITF. Depending on a short interspersed nucleotide element (SINE), the dog is spotted or not.
Del is the dominant allele and causes solid or single-coloured dogs (genotype del/del). Dogs that inherit SINE (genotype int/int) homozygous have white markings that either cover at least the ventral surface (mantle pattern) or most of the body (piebald or extreme white spotting). In most breeds, dogs heterozygous for the SINE-insertion (genotype del/int) are solid colored or have minimal white spots, e.g. on the toes. In some other breeds heterozygotes show white undersides, often with a white collar called pseudo-Irish.
* this test will be performed by a partner lab
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Inheritance :
trait
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. .
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7 ) I - Locus (phaeomelanin intensity)
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Breeds
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Afghan Hound
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Japanese Akita Inu
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Alaskan Malamute
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All Dog Breeds
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Australian Shepherd
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Australian Silky Terrier
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Bichon Frise
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Coton de Tulear
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Curly Coated Retriever
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Elo
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Eurasier
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French Bull Dog
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German Shepherd
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Great Dane
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Hungarian Pulis (Hungarian water dog)
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Huskies
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Leonberger
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Poodle
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Pug
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Soft Coated Wheaten Terrier
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Saluki
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Samoyed
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Schnauzer
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Australian Silky Terrier
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West Highland White Terrier
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WhiteS Swiss Shepherd
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Description |
I-Locus controls the intensity of the red pigment (phaeomelanin). Phaeomelanin is the tan pigment including all shades of red, gold, fawn, sable and cream pigments. The richness of the red colour varies in the different breeds and within a breed, from the very rich red of the Irish setter to cream. This intensity is controlled by the I-Locus which has recently been identified.The dominant I-allele stands for intense phaeomelanin (red, orange, yellow), the recessive i-allele is responsible for for cream, cream-white or white.
The expression of the phaeomelanin on the dog body, is initially determined by the loci E, K and A locus. The I-Locus decides how bright or rich the red colour is.
A test is now available at Laboklin.
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Sample Requirements |
Whole blood in EDTA tube (0.5 - 1 ml) or Buccal swabs. .
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Price
for the above 7 tests
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£ 156.00 (including VAT)
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To order:
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Download
Order Form from this link 
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Complete the order form and send it together
with your samples to the following address:
Laboklin (UK), 125 Northenden Road, Manchester, M33 3HF
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