This test is for the special E-Locus colour variants 'eA', 'eG' and 'eH'.
'eA': Hare-Pied / Husky Marking
The 'eA' variant is located a the E-Locus, it is recessive to 'EM' and 'E' but dominant to 'e'. This variant restricts the production of eumelanin. In dogs which are genetically black and tan (K-Locus: ky/ky and A-locus: at/at or at/a or in Pomeranian aw/-) the presence of the eA allele variant (eA/e or eA/eA) will lead to the expression of the husky marking pattern because it makes any area with solid eumelanin ((black / brown / blue pigments)) smaller while causing bigger phaeomelanin (Yellow pigment / fawn / sable) markings. In Beagle it will lead to the Harepid pattern, this is due to the saddle tan. The eumelanin of the black saddle will be reduced to black tips on the hairs down the middle of the dog's back,a hare-pied dog may have dark rims on its nose, but the center part is pale, which is also known as a “butterfly-nose.” Beagle harepied puppies look like tan/whites when they are born but with dark eye rims.
in dogs which have KB/- at the K-locus , the eA variant (eA/e or eA/eA) will prevent the expresion of the dominant black and so the expressed colour will be sololey expressed by the A-Locus.
'eG': Domino (Afgan Hound) / Grizzle (Saluki) / Sable (Borzoi)
The 'eG' allele encodes a coat colour variant called 'domino' in Afghan hounds ,'grizzle' in Salukis. This pattern is characterized by a pale face with a widow’s peak above the eyes and appears to be unique to these two old dog breeds. In Borzoi it causes the 'sable' pattern
A typical domino sighthound would have eG/eG or eG/e at the E-Locus, at/at genotype at the A locus and ky/ky at the K-locus.
if the E-Locus is eG/eG or eG/e, and the A-Locus is at/at but the K-Locus is KB/KB or Kb/ky the dog would have a so called 'domino black' which is characterised by grey streaked coat colour.
if the E-Locus is eG/eG or eG/e, the K-Locus is ky/ky but the A-Locus is AY/AY or AY/at the coat colour would be crème yellow or crème red without dark hair tips.
Dogs with N/eG at the E-locus may exhibit exhibit some hints that they carry the eG variant, and it the dog has 'ky/ky' at the K=Locus and 'at/at' at the A-Locus, tan marking will be larger than normal
'eH' Sable in Cocker Spaniels
Sable is a coat colour variant in English and American Cocker Spaniels, in which the expression of eumelanin is partially inhibited. The typical markings are a pale mask and a dark blanket. A mutation at the EH-locus, located between E and e, is responsible for this phenotype. Sable Cocker Spaniels would have eH/eH or eH/e at the E-locus and KB/ky or KB/KB at the K-Locus.
if the E-Locus is eH/eH or eH/e, the K-Locus is ky/ky the coat colour have been reported as 'dirty red' not sable.
do not confuse this sable with the usual AY sable at the A-Locus. The A-Locus is all Cocker Spaniels is fixed for at/at and there is no point testing the A-Locus in this breed.
important note 1
Please note that when you order this test if the breed is is Afgan Hound, Borzoi and Saluki we will only analyse the 'eG' variant, if the breed is Cocker Spaniels, we will only analyse the 'eH' variant, in all other breeds including Beagle, we will only analyse the 'eA' variant.
important note 2
Please note that this test does not detect the 'e1' allele, for the e1 allele, you also need to order the
E-Locus (e1) test available on this link for a complete analysis. You can take advantage of our offer 'every additional coat colour test is charged at half the price'
