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ELISA
Saliva collection instructions for cortisol testing in guinea pigs
Indication:
Therapy monitoring for Cushing’s syndrome.
Using Salivette® Cortisol is recommended to ensure a sufficient saliva volume of at least 50 µl for reliable cortisol measurement.
Preparation
- Ensure the guinea pig is calm and gently handled to minimise stress-related cortisol changes.
- Rinse the mouth with a small amount of water to remove any feed particles.
Collection
- Wait 30 minutes after rinsing to allow saliva to regenerate and avoid dilution of the sample.
- Offer the fixed synthetic fibre roll from the Salivette and allow the guinea pig to chew on it until it is well moistened.
- Place the Salivette back into its tube and centrifuge to extract the saliva.
Test procedure
- Material: 2 x saliva 0.1 ml each (sample containers (Salivettes®) will be provided)
- 1st saliva sample collection baseline cortisol
- injection of 2 IU ACTH/kg bdw, i.v./i.m.
- 2nd sample collection after 4 hours
Storage and shipping
- Transfer the collected saliva to a labelled tube if required.
- Keep the sample chilled and ship it with ice packs to maintain stability during transport.
If treatment is adequately controlled, no or only a minimal increase in
cortisol concentration is observed.
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